X-ray Imaging of Zinc Uptake and Accumulation in Hyperaccumulator Thlaspi caerulescens

نویسنده

  • Megan L. Toaspern
چکیده

The use of high energy x-ray fluorescence as a trace metal probe offers scientists an innovative tool for research. One application is the study of heavy metal accumulation in plant systems. The test species Thlaspi caerulescens has the ability to accumulate massive amounts of zinc, cadmium, and lead from contaminated soils as a form of bioremediation. In order to understand the efficiency of the plant, the distribution and relative concentration of zinc was determined for the hyperaccumulator Thlaspi caerulescens and a similar plant, Thlaspi arvense, a nonaccumulator. The leaves of the Thlaspi plants were scanned using x-ray microprobe fluorescence. An elemental distribution map of zinc concentration in the plant leaf was generated. Thlaspi caerulescens distributed zinc throughout the tissue of the plant leaf while Thlaspi arvense accumulated zinc in the veins of the plant with little translocation of zinc to surrounding tissues.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Reciprocal grafting separates the roles of the root and shoot in zinc hyperaccumulation in Thlaspi caerulescens

* The extreme phenotype of zinc (Zn) hyperaccumulation, which is found in several Brassicaceae species, is determined by mechanisms that promote elevated Zn tolerance and high Zn accumulation in shoots. * We used reciprocal grafting between a Zn hyperaccumulator, Thlaspi caerulescens, and a Zn nonaccumulator, Thlaspi perfoliatum, to determine the relative importance of roots and shoots in Zn hy...

متن کامل

Transport and detoxification of cadmium, copper and zinc in the Cd/Zn hyperaccumulator plant Thlaspi caerulescens

Hyperaccumulators store most of the accumulated metal in the vacuoles of large leaf epidermal cells (storage cells). For investigating cadmium uptake, we incubated a protoplast mixture obtained by digestion of leaves of Thlaspi caerulescens (Ganges ecotype) with a Cd-specific fluorescent dye. A fluorescence kinetic microscope was used for selectively measuring Cd-uptake and photosynthesis in di...

متن کامل

The molecular physiology of heavy metal transport in the Zn/Cd hyperaccumulator Thlaspi caerulescens.

An integrated molecular and physiological investigation of the fundamental mechanisms of heavy metal accumulation was conducted in Thlaspi caerulescens, a Zn/Cd-hyperaccumulating plant species. A heavy metal transporter cDNA, ZNT1, was cloned from T. caerulescens through functional complementation in yeast and was shown to mediate high-affinity Zn(2+) uptake as well as low-affinity Cd(2+) uptak...

متن کامل

Cellular compartmentation of zinc in leaves of the hyperaccumulator thlaspi caerulescens

Cellular compartmentation of Zn in the leaves of the hyperaccumulator Thlaspi caerulescens was investigated using energy-dispersive x-ray microanalysis and single-cell sap extraction. Energy-dispersive x-ray microanalysis of frozen, hydrated leaf tissues showed greatly enhanced Zn accumulation in the epidermis compared with the mesophyll cells. The relative Zn concentration in the epidermal cel...

متن کامل

Transcription profiling of the metal-hyperaccumulator Thlaspi caerulescens (J. & C. PRESL).

Thlaspi caerulescens is a well-studied metal-hyperaccumulator of zinc, cadmium and nickel, belonging to the Brassicaceae family. Moreover it is one of the few hyperaccumulators that occur on different metalliferous soil types, as well as on nonmetalliferous soils. We are interested in the development of systems to improve phytoremediation of metal contaminated soils through improved metal-accum...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 1999